Effects of Different Pretreatment Methods on Seed Germination of Rhododendron Aureum and R． Parvifolium

27 Mar Effects of Different Pretreatment Methods on Seed Germination of Rhododendron Aureum and R． Parvifolium

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Abstract

English Abstract

Seeds of Rhododendron aureum Georgi and R． parvifolium Admas． were pretreated by three methods including 100，200，400，600 and 800 mg·L－1 GA3 soaking for 24 h，water soaking for 6，12 and 24 h，ultrasonic wave treating for 10，20 and 30 min． And taking seeds without pretreatment as the control，seed germination status of two species were comparatively analyzed by means of six analysis indexes including germination rate，germination energy，germination index and delay time，peak period and lasting time of germination． The results show that suitable pretreatment methods have an effective promotion role in seed germination of two species． Generally， the promotion role of GA3 soaking is better than that of the other methods． Germination rate，germination energy and germination index of two species seeds soaked with different concentrations of GA3 for 24 h are all extremely significant higher and delay time and peak period of germination are shorter than those of the control． In which，germination rates of R． aureum seeds soaked with 400 mg·L－1 GA3 and R． parvifolium seeds soaked with 600 mg·L－1 GA3 are the highest with percentage of 73． 50% and 81． 00%，respectively． Germination rates of R． aureum seeds soaked for 6 h with water and R． parvifolium seeds soaked for 24 h with water
are significantly and extremely significantly higher than those of the control， respectively． After treated for 10 or 20 min with ultrasonic wave ( 50 W，40 kHz) ，germination rate of two species seeds is obviously significantly higher than that of the control， in which， that of R． aureum treated for 20 min and that of R． parvifolium treated for 10 min are the highest． But effects of water soaking and ultrasonic wave treatment on delay time，peak period and lasting time of germination are not obvious． It is suggested that the best pretreatment method to R． aureum and R． parvifolium seeds is soaked for 24 h with 400 mg·L－1 GA3 and with 600 mg·L－1 GA3， respectively，which can extremely significantly improve germination rate and shorten germination time．