Embryo Germination and Rapid Propagation of Rare Andendangered Plant Davidia Involucrata

27 Mar Embryo Germination and Rapid Propagation of Rare Andendangered Plant Davidia Involucrata

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Abstract

首次采用均匀设计和回归分析方法对影响珙桐(Davidia involucrata)胚萌发的3种因素(基本培养基、赤霉素和活性 炭)进行优化, 并利用萌发胚建立了珙桐的高效再生体系。 利用回归方程得出胚萌发的最优条件为: 在含有0.5 mg.L-1 IBA和 1 mg.L-1 6-BA的12MS培养基中添加2.85 mg.L-1 GA3和0.25 g.L-1活性炭。在验证实验中珙桐胚萌发率为87.72%, 与预测 值无显著差异。 最优的芽诱导与增殖条件为: 添加0.1 mg.L-1 NAA和2.0 mg.L-1 6-BA 的WPM培养基, 增殖系数为4.34。在 生根培养基中增殖的芽生根率高达90%, 移栽成活率为71%。

English Abstract

Three factors (media type, gibberellic acid [GA3] and active carbon) affecting the embryo germination of Davidia
involucrata were optimized by use of a uniform design and analyzed by regression analysis. Then a system for micropropagating
D. involucrata was established from the embryos. From regression equations, the optimal conditions for embryo germination were
the addition of 2.85 mg.L-1 GA3 and 0.25 g.L-1 activated carbon to 12 MS medium containing 0.5 mg.L-1 IBA and 1 mg.L-1 6-BA. The
germination rate of embryo was 87.72%, without significant deviation to the predicted value. The optimal conditions for shoot
induction and multiplication were WPM medium supplemented with 0.1 mg.L-1 NAA and 2.0 mg.L-1 6-BA; the mean shoots obtained
per explant was 4.34. An amount of 90% of shoots rooted on rooting medium, and 71% plants survived after transplanting.