Estimating Population Size and Genetic Diversity of Asian Elephant in The shangyong Nature Reserve

27 Mar Estimating Population Size and Genetic Diversity of Asian Elephant in The shangyong Nature Reserve

View Original

Abstract

亚洲象(Elephas maximus)是我国国家一级保护动物, 准确地评估其种群大小和遗传多样性对该物种的保护 具有十分重要的意义。本文采用非损伤性取样方法, 并结合野外观察数据对尚勇保护区当前亚洲象种群数量进行 了评估, 同时对该种群的遗传多样性进行了分析。采用7对微卫星引物对185份粪便样品进行扩增, 通过基因分型, 得到59个独特的基因型。文章采用两种方法评估种群数量, 即聚集曲线法和分子标记重捕法, 估计尚勇保护区的 亚洲象种群数量为76 ± 8头(95%置信区间内为67–99头)。遗传多样性方面, 平均等位基因数为3.86, 平均观察杂合 度为0.52, 平均期望杂合度为0.42, 平均多态信息含量为0.34, 表明尚勇保护区亚洲象种群遗传多样性水平为中等 偏低, 与勐养种群遗传多样性水平相似。

English Abstract

Asian elephant (Elephas maximus) is a first class protected species in China. Estimation of the
population size and genetic variability is crucial to the management and conservation of this species. A genetic survey was conducted using multi locus genotyping of noninvasively collected fecal samples of the
Asian elephant population in the Shangyong Nature Reserve, Yunnan Province of China. Amplification of
185 fecal DNA samples by 7 microsatellite primers resulted in a total of 59 unique genotypes. The average
number of alleles was 3.86, the average observed and expected heterozygosity and polymorphism information content (PIC), was 0.52, 0.42 and 0.34, respectively. Accumulation curve method and capture mark
re-capture were used to evaluate the population size. Results indicated that the population size in Shangyong
Nature Reserve was 76 ± 8 (from 67 to 99 individuals, 95% confidence interval). Overall, a moderate level of
genetic variability was observed in the Shangyong population, which was similar to a population in the Mengyang region