Form and Embryonic Characteristics of Pedicularis Hallaisanensis Seeds as Endangered Wild Species Ii-class Using Host Plants

27 Mar Form and Embryonic Characteristics of Pedicularis Hallaisanensis Seeds as Endangered Wild Species Ii-class Using Host Plants

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Abstract

본 연구는 멸종위기야생식물 Ⅱ급인 한라송이풀(Pedicularis hallaisanensis)을 대상으로 종자를 통한 번식체계 확립 가능성을 조사하기 위해 수행하였다. 한라송이풀의 종자 형태는 난형이고, 종피는 진갈색을 띠고 있다. 종자의 단면을 잘라 확인한 배는 왜소형(Dwarf type)이었다. 종자의 길이는 평균(±표준편차) 0.47±0.07 ㎜이고, 너비는 0.16±0.006 ㎜, 두께는0.12±0.01 ㎜로 나타났다. 종자 1립의 무게는 0.0003±0.0001 ㎎이며, 천립중은 4.59±0.02 ㎎으로 나타났다. Tetrazolium (TZ) 검정에 의한 한라송이풀 종자의 활력도는 75.33%로 나타났다. 한라송이풀을 4주간 4℃에 저장한 후의 발아율은 71%로 가장높았고, 6주간 처리 64%, 8주간 처리 60%로 나타나 저장 기간이 증가할수록 발아율은 낮아지는 경향을 보였다. 한라송이풀 종자에 영향을 미치는 숙주식물의 영향은 쑥에서 파종 후 53.5일에, 구절초는 62.5일에 최초로 발아를 하였다. 두 숙주식물에서 164일 이후에는 발아한 종자를 확인하지 못하였다. 쑥을 사용한 경우, 한라송이풀의 발아율은 평균 45.5%, 구절초는 19.5%로 나타났다. 평균 발아일수는 쑥에서 70.2일, 구절초에서는 46.8일로 나타났다.

English Abstract

This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was 0.47 ± 0.07 mm, width 0.16 ± 0.006 mm, and thickness 0.12 ± 0.01 mm. The weight of one seed was 0.0003 ± 0.0001 mg, and 1000 seeds weighed 4.59 ± 0.02 mg. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at 4°C. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at 4°C were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination.
The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.